Nucleic Acids Chemistry
KOHJI SEIO's group
Tokyo Institute of Technology
Department of Life Science and Technology
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Recent publications
Synthesis of photocaged 6-O-(2-nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl) uridine triphosphates for photocontrol of the RNA transcription reaction.
Ohno K, Sugiyama D, Takeshita L, Kanamori T, Masaki Y, Sekine M, Seio K.
Bioorg Med Chem. 2017 Sep 21. pii: S0968-0896(17)31538-9. doi: 10.1016/j.bmc.2017.09.032.




Synthesis of oligonucleotides containing 2-N-heteroarylguanine residues and their effect on duplex/triplex stability.
Inde T, Masaki Y, Maruyama A, Ito Y, Makio N, Miyatake Y, Tomori T, Sekine M, Seio K.
Org Biomol Chem. 2017 Oct 11;15(39):8371-8383. doi: 10.1039/c7ob01875d.



A Systematic Study of the Synthesis of 2ʹ-Deoxynucleosides by Mitsunobu Reaction.
Seio K, Tokugawa M, Kaneko K, Shiozawa T, Masaki, Y.
Synlett 2017; 28(15): 2014-2017. doi: 10.1055/s-0036-1588445.



Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes.
Masaki Y, Sekine M, Seio K.
Curr Protoc Nucleic Acid Chem. 2017 Mar 2;68:7.27.1-7.27.10.
doi:10.1002/cpnc.25.

Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation.
Kanamori T, Takamura A, Tago N, Masaki Y, Ohkubo A, Sekine M, Seio K.
Org Biomol Chem. 2017 Feb 1;15(5):1190-1197. doi:10.1039/c6ob01278g.

Enzymatic synthesis and reverse transcription of RNAs incorporating 2′-O-carbamoyl uridine triphosphate
Masaki Y, Ito H, Oda Y, Yamazaki K, Tago N, Ohno K, Ishii N, Tsunoda H, Kanamori T, Ohkubo A, Sekine M, Seio K.
Chem Commun (Camb). 2016 Oct 25;52(87):12889-12892. doi:10.1039/C6CC05796A

Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine
Seio K, Ohno Y, Ohno K, Takeshita L, Kanamori T, Masaki Y, Sekine M.
Bioorg Med Chem Lett. 2016 Oct 1;26(19):4861-3. doi: 10.1016/j.bmcl.2016.07.075.

An Effective Strategy for a Conformer-Selective Detection of Short-Lived Excited State Species: Application to the IR Spectroscopy of the N1H Keto Tautomer of Guanine
Asami H, Tokugawa M, Masaki Y, Ishiuchi S, Gloaguen E, Seio K, Saigusa H, Fujii M, Sekine M, Mons M.
J Phys Chem A. 2016 Apr 14;120(14):2179-84. doi: 10.1021/acs.jpca.6b01194.

7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein
Tokugawa M, Masaki Y, Canggadibrata J C, Kaneko K, Shiozawa T, Kanamori T, Grøtli M, Wilhelmsson L M, Sekine M, Seio K.
Chem Commun (Camb). 2016 Mar 7;52(19):3809-12. doi: 10.1039/c5cc09700b.

Synthesis of 5-[3-(2-aminopyrimidin-4-yl)aminopropyn-1-yl]uracil derivative that recognizes Ade-Thy base pairs in double-stranded DNA
Ito Y, Masaki Y, Kanamori T, Ohkubo A, Seio K, Sekine M.
Bioorg Med Chem Lett. 2016 Jan 1;26(1):194-6. doi: 10.1016/j.bmcl.2015.11.003.

2015
A New Microfluidic Phase-Transfer Reaction Using HPLC Guard Columns as the Reactor for the N3-Protection of Uridine Derivatives
Tago N, Masaki Y, Nagasawa H, Kanamori T, Ohkubo A, Seio K, Sekine M.
Synlett. 2015 26(18): 2578-2582. doi:10.1055/s-0035-1560264

Design, Synthesis, and Properties of Phosphoramidate 2′,5′-Linked Branched RNA: Toward the Rational Design of Inhibitors of the RNA Lariat Debranching Enzyme
Tago N, Katolik A, Clark N E, Montemayor E J, Seio K, Sekine M. Hart P J, Damha M J.
J Org Chem. 2015 Oct 16;80(20):10108-18. doi: 10.1021/acs.joc.5b01719.

Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs
Ohkubo A, Yamada K, Ito Y, Yoshimura K, Miyauchi K, Kanamori T, Masaki Y, Seio K, Yuasa H, Sekine M.
Nucleic Acids Res., 2015 May 3;43:5675-5686. doi:10.1093/nar/gkv496

Synthesis of Peptide Nucleic Acids Containing Pyridazine Derivatives As Cytosine and Thymine Analogs, and Their Duplexes with Complementary Oligodeoxynucleotides
Tomori T, Miyatake Y, Sato Y, Kanamori T, Masaki Y, Ohkubo A, Sekine M, Seio K.
Org. Lett., 2015 Mar 10;17:1609–1612. doi:10.1021/acs.orglett.5b00522

Enhancement of exon skipping in mdx52 mice by 2´-O-methyl- 2-thioribothymidine incorporation into phosphorothioate oligonucleotides
Masaki Y, Inde T, Nagata T, Tanihata J, Kanamori T, Seio K, Takeda S, Sekine M.
Med. Chem. Commun., 2015 Apr 1;6:630-633. doi:10.1039/C4MD00468J

Synthesis of Responsive Fluorescent Nucleobases 7-(Benzofuran-2-yl)-7-deazahypoxanthine and 7-(Benzofuran-2-yl)-7-deazaguanine using Cross-coupling Reaction
Tokugawa M, Kaneko K, Saito M, Kanamori T, Masaki Y, Ohkubo A, Sekine M, Seio K.
Chem Lett. 2015 Jan 5;44:64-66. doi:10.1246/cl.140879

Controlling the Fluorescence of Benzofuran-Modified Uracil Residues in Oligonucleotides by Triple-Helix Formation
Kanamori T, Ohzeki H, Masaki Y, Ohkubo A, Takahashi M, Tsuda K, Ito T, Shirouzu M, Kuwasako K, Muto Y, Sekine M, Seio K.
ChemBioChem. 2015 Jan 2;16:167-176. doi:10.1002/cbic.201402346

Synthesis and properties of oligonucleotides modified with 2´-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives
Yamada T, Masaki Y, Okaniwa N, Kanamori T, Ohkubo A, Tsunoda H, Seio K, Sekine M.
Org Biomol Chem. 2014 Jun 27;12(33):6457-6464 doi:10.1039/C4OB01260G


A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds
Yamada K, Masaki Y, Tsunoda H, Ohkubo A, Seio K, Sekine M.
Org Biomol Chem. 2014 Feb 11;12(14):2255-2262 doi:10.1039/C3OB42420K


Properties of 5- and/or 2-modified 2´-O-cyanoethyl uridine residue : 2´-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component
Masaki Y, Miyasaka R, Hirai K, Kanamori T, Tsunoda H, Ohkubo A, Seio K, Sekine M.
Org Biomol Chem. 2014 Feb 21;12(7):1157-1162 doi:10.1039/C3OB41983E


Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region
Seio K, Tokugawa M, Tsunoda H, Ohkubo A, Arisaka H, Sekine M.
Bioorg Med Chem Lett. 2013 Dec 15;23(24):6822-6824. doi: 10.1016/j.bmcl.2013.10.012.


Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus.
Iijima Y, Kojima S, Kodama E, Kurohagi S, Kanamori T, Masaki Y, Ohkubo A, Sekine M, Seio K.
Org Biomol Chem. 2013 Oct 2;11(47):8276-8282 doi:10.1039/C3OB41901K


Chemical synthesis of U1 snRNA derivatives.
Ohkubo A, Kondo Y, Suzuki M, Kobayashi H, Kanamori T, Masaki Y, Seio K, Nagai K, Sekine M.
Org Lett. 2013 August 16;15(17):4386-4389 doi:10.1021/ol401917r


Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine.
Seio K, Kanamori T, Tokugawa M, Ohzeki H, Masaki Y, Tsunoda H, Ohkubo A, Sekine M.
Bioorg Med Chem. 2013 June 1;21(11):3197-3201. doi: 10.1016/j.bmc.2013.03.034.


Base recognition of gap sites in DNA–DNA and DNA–RNA duplexes by short oligonucleotides.
Yamada K, Ohkubo A, Esaka Y, Kanamori T, Masaki Y, Seio K, Sekine M.
Bioorg Med Chem Lett. 2013 June 1;23(11):3448-3451. doi: 10.1016/j.bmcl.2013.03.054.


Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups.
Yamada K, Hattori Y, Inde T, Kanamori T, Ohkubo A, Seio K, Sekine M.
Bioorg Med Chem Lett. 2013 Feb 1;23(3):776-778. pii: S0960-894X(12)01531-4. doi: 10.1016/j.bmcl.2012.11.079.